Synthesis and characterization of Silver nanoparticles from Cinnamomum tamala leaf extract and its antibacterial potential

Document Type: Reasearch Paper

Authors

1 Department of Applied Chemistry and Chemical Engineering, Islamic University, Kushtia 7003, Bangladesh.

2 Senior Scientific Officer, Chemical Research Division, BCSIR Laboratories, Dhaka-1000, Bangladesh.

3 Senior Scientific Officer, Institute of Fuel Research Division, BCSIR Laboratories, Dhaka-1000, Bangladesh.

Abstract

The novel approach has been carried for the green synthesis of silver nanoparticles using leaf extract of Cinnamomum tamala and silver nitrate solution. The optimal condition for synthesizing Ag-NPs was obtained by varying the leaf extract concentration, temperature, AgNO3 concentration, effect of ratio of leaf extract to AgNO3 solution,pH and reaction time. The formation of silver nanoparticles was confirmed by UV–Vis spectrophotometer. Fourier transform infrared spectroscopy (FTIR) was used to key out the specific functional groups responsible for the reduction of silver nitrate to form silver nanoparticles and the capping agents present in the leaf extract. Scanning electron microscopy (SEM) represents the morphological characterization of synthesized nanomaterials. Transmission electron microscopy (TEM) analysis revealed that the particles were crystalline, spherical and irregular in shape and the size were 16 nm and 9 nm at 25 °C and 60 °C, respectively. Electron X-ray diffraction (XRD) analysis is used to determine the phase distribution, crystallinity and purity of the synthesized nanoparticles. The synthesized nanoparticles are found to be highly effective against some pathogenic bacteria species, thus signification of the present study is in production of various pharmaceutical and bioactive products.

Keywords

Main Subjects


[1] Bangale S., Ghotekar S., (2019), Bio-fabrication of Silver nanoparticles using Rosa Chinensis L. extract for antibacterial activities. Int. J. Nano Dimens. 10: 217-224.

[2] Xu Z. P., Zeng Q. H., Lu G. Q., Yu A. B., (2006), Inorganic nanoparticles as carriers for efficient cellular delivery. Chem. Eng. Sci. 61: 1027- 1040. 

[3] Pal S., Tak Y. K., Song J. M., (2007), Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram negative bacterium Escherichia coli. Appl. Environ. 73: 1712-1720.

[4] Parashar U. K., Saxena S. P., Srivastava A., (2009), Bioinspired synthesis of silver nanoparticles. Dig. J. Nanomat. Biosynth. 4: 159-166.

[5] Wijnhoven S. W. P., Peijnenburg W. J. G. M., Herberts C. A., Hagens W. I., Oomen A. G., Heugens E. H. W., (2009), Nano-silver: A review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicol. 3: 109-138.

[6] Lee K. S., El-Sayed M. A., (2006), Gold and silver nanoparticles in sensing and imaging: Sensitivity of plasmon response to size, shape, and metal composition. J. Phys. Chem. B. 110: 19220-19225.

[7] Jain P. K., Huang X., El-Sayed I. H., EL-Sayed M. A., (2008), Noble metals on the nanoscale: Optical and photothermal properties and some applications in imaging, sensing, biology, and medicine. Acc. Chem. Res. 41: 1578-1586.

[8] Nair L. S., Laurencin C. T., (2007), Silver nanoparticles: Synthesis and therapeutic applications. J. Biomed. Nanotechnol. 3: 301-316.

[9] Alizadeh H., Salouti M., Shapouri R., (2013), Intramacrophage antimicrobial effect of silver nanoparticles against Brucella melitensis 16M. Sci. Iranica. 20: 1035-1038.

[10] Wong K. K., Cheung S. O., Huang L., Niu J., Tao C., Ho C. M., Che C. M., Tam P. K., (2009), Further evidence of the antiinflammatory effects of silver nanoparticles. Chem. Med. Chem. 4: 1129-1135.

[11] Rawani A., Ghosh A., Chandra G., (2013), Mosquito larvicidal and antimicrobial activity of synthesized nano-crystalline silver particles using leaves and green berry extract of Solanum nigrum L. (Solanaceae: Solanales). Acta Trop. 128: 613-622.

[12] Nadaroglu H., Alayli Gungor A., Ince S., (2017), Synthesis of nanoparticles by green synthesis  method. Int. J. Innov. Res. Rev. 1: 6-9.

[13] Kharissova O. V., Dias H. V. R., Kharisov B. I., Perez B. O., Perez V. M. J., (2013), The greener synthesis of nanoparticles. Trends in Biotech. 31: 240-248.

[14] Devi S. L., Kannappan S., Anuradha C. V., (2007), Evaluation of in vitro antioxidant activity of Indian bay leaf, Cinnamomum tamala (Buch.-Ham.) T. Nees & Eberm using rat brain synaptosomes as model system. Indian J. Exp. Biol. 45: 778-784.

[15] Chanotiya C. S., Yadav A., (2010), Enantioenriched (3S)-(+)-linalool in the leaf oil of Cinnamomum tamala Nees et Eberm. from Kumaon. J. Ess. Oil Res. 22: 593-596.

[16] Arunachalam R., Dhanasingh S., Kalimuthu B., Uthirappan M., Rose C., Mandal A. B., (2012), Phytosynthesis of silver nanoparticles using Coccinia grandis leaf extract and its application in the photocatalytic degradation. Colloids and Surf. Biointerf. 94: 226-230.

[17] Parekh J., Chanda S. V., (2008), In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk. J. Biotechnol. 31: 53-58.

[18] Guruvaiah P., Arunachalam A., Velan L. P. T., (2012), Evaluation of phytochemical constituents and antioxidant activities of successive solvent extracts of leaves of Indigofera caerulea Roxb using various in vitro antioxidant assay systems. Asian Pacific J. Tropical Dis. 2: 118-123.

[19] Rakholiya K., Chanda S., (2012), In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains. Asian Pac. J. Trop. Biomed. S876–S880.

[20] Mahitha B., Deva P., Raju B., Dillip G. R., Reddy C. M., Mallikarjuna K., Manoj L., Priyanka S., Rao K. J., Sushma N. J., (2011), Biosynthesis, characterization and antimicrobial studies of Ag NPs extract from Bacopa monniera whole plant. Dig. J. Nanomat. Biosynth. 6: 135-142.

[21] Vijayaraghavan K., Nalini S. P. K., Prakash N. U., Madhankumar D., (2012), Biomimetic synthesis  of silver nanoparticles by aqueous extract of Syzygium aromaticum. Mat. Lett.75: 33-35.

[22] Rajoriya P., (2017), Green synthesis of silver nanoparticles, their characterization and antimicrobial potential. Phd thesis, Sam Higginbottom University of Agriculture, Technology & Sciences.

[23] Song J. Y., Kim B. S., (2009), Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioproc. Biosyst. Eng. 32: 79-84.

[24] Kaviya S., Santhanalakshmi J., Viswanathan B., Muthumary J., Srinivasan K., (2011), Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochim. Acta A: Mol. Biomol. Spectrosc. 79: 594-598.

[25] Sathishkumar M., Sneha K., Won S. W., Cho C. W., Kim S., Yun Y. S., (2009), Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids and Surf. B: Biointerf. 73: 332-338.

[26] Philip D., (2010), Green synthesis of gold and silver nanoparticles using Hibiscus rosasinensis. Physica E: Low-Dimens. Sys. Nanostruc. 42: 1417-1424.

[27] Aswathy S., Philip D. A., (2012), Facile one-pot synthesis of gold nanoparticles using tannic acid and its application in catalysis. Phys. E. 44: 1692–1696.

[28] Srivastava A. A., Kulkarni A. P., Harpale P. M., Zunjarrao R. S., (2011), Plant mediated synthesis of silver nanoparticles using a bryophyte: Fissidens minutes and its anti-microbial activity. Int. J. Eng. Sci. Tech. 3: 8342-8347.

[29] Tripathy A., Raichur A. M., Chandrasekaran N. P., Mukherjee A., (2010), Process variables in biomimetic synthesis of silver nanoparticles by aqueous extract of Azadirachta indica (Neem) leaves. J. Nanopart. Res. 12: 237- 246.

[30] Padalia H., Moteriya P., Chanda S., (2015), Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arab. J. Chem. 8: 732-741.

[31] Kokila T., Ramesh P. S., Geetha D., (2015), Biosynthesis of silver nanoparticles from cavendish banana peel extract and its antibacterial and free radical scavenging assay: A novel biological approach. App. Nanosci. 5: 911–920.

[32] Sankar R., Rizwana K., (2015),Ultra-rapid photocatalytic activity of Azadirachta indica engineered colloidal titanium dioxide nanoparticles. App. Nanosci. 5: 731–736.

[33] Pant G., Nayak N., Prasuna R. G., (2012), Enhancement of antidandruff activity of shampoo by biosynthesized silver nanoparticles from Solanum trilobatum plant leaf. App. Nanosci. 3: 431-439.

[34] Roopan S. M., Rohit M. G., Rahuman A. A., Kamaraj C., Bharathi A., Surendra T. V., (2013), Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Ind. Crops. Prod. 43: 631-635.

[35] Fatema S., Shirsat M., Farooqui M., Pathan M. A., (2019), Biosynthesis of Silver nanoparticle using aqueous extract of Saraca asoca leaves, its characterization and antimicrobial activity. Int. J. Nano Dimens. 10: 163-168.

[36] Chanda S., (2013), Silver nanoparticles (medicinal plants mediated): A new generation of antimicrobials to combat microbial pathogens-a review. In MendezVilas, A. (Ed). Microbial pathogens and strategies for combating them: science, technology and education, pp. 1314-1323. Spain:Formatex.

[37] Kumar D. A., Kumar V., Palanichamy S. M., Roopan S. M., (2014), Green synthesis of silver nanoparticles using Alternanthera dentata leaf extract at room temperature and their antimicrobial activity. Spectrochim. Acta Part A-Mol. Biomol. Spectros. 127: 168-171.

[38] Sivakumar J., Kumar C. P., Santhanam P., Saraswathi N., (2011), Biosynthesis of silver nanoparticles using Calotropis gigantean Leaf. Afr. J. Basic Appl. Sci. 3: 265-270.

[39] Venu R., Ramulu T. S., Anandakumar S., Rani V. S., Kim C. G., (2011), Bio-directed synthesis of platinum nanoparticles using aqueous honey solutions and their catalytic applications. Colloids and Surf. A: Physicochem. Eng. Aspects. 384: 733-738.

[40] Yilmaz M., Turkdemir H., Kilic M. A., Bayram E., Cicek A., Mete A., Ulug B., (2011), Biosynthesis of silver nanoparticles using leaves of Stevia rebaudiana. Mater. Chem. Phys. 130: 1195-1202.