Synthesis of MnFe2O4 nanoparticles for adsorption of digestive enzymes: Kinetics, isothermal and thermodynamics studies

Document Type : Reasearch Paper


1 Chemistry Department, Federal University of Agriculture, Abeokuta, Nigeria.

2 Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria.


The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of amylase and protease on to manganese ferrite (MnFe2O4). The metal ferrite was synthesized via a sol – gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnetic resonance (EPR) and Fourier transform infrared spectroscopy (FTIR). The adsorption was study in a batch process and the data were subjected to kinetics and isotherm models. The pseudo-first order best fitted the kinetic data with R2> 0.99. The data were fitted well by the entire isotherm models considered with the maximum adsorption isotherm of 1.602 and 7.330 mg/g.  The thermodynamic parameters give negative Gibb’s free energy, ΔG, showing a spontaneous adsorption; positive ΔH indicated an endothermic favoured process, while ΔS values showed that the process progress with lower entropy change.


Main Subjects

  1. Felsovalyi F., (2012), Mechanistic study of the adsorption and desorption of proteins on silica, PhD Thesis, Colombia University p.218.
  2. Dolatshahi-Pirouz A., Rechendorff K., Hovgaard M. B., Foss M., Chevallier J., Besenbacher F., (2008), Bovine serum albumin adsorption on nano-rough platinum surfaces studied by QCM-D. Colloids and Surf. B: Biointerf. 66: 53–59.
  3. Long D., Zhang R., Qiao W., Zhang L., Liang X., Ling L., (2009), Biomolecular adsorption behavior on spherical carbon aerogels with various mesopore sizes. J. Colloid and Interf. Sci. 331: 40–60.
  4. Mavropoulos E., Costa A. M., Costa L. T., Achete C. A., Mello A., Granjeiro J. M., Rossi A., (2011), Adsorption and bioactivity studies of albumin onto hydroxyapatite surface. Colloids and Surf. B: Biointerf. 83: 1–9.
  5. Bozgeyik K., Kopac T., (2016), Adsorption properties of arc produced multi walled carbon nanotubes for bovine serum Albumin. Int. J. Chem. Reactor Eng. 14: 549–558.
  6. Hartman M., (2015), Ordered mesoporous materials for bioadsorption and biocatalysis. Chem. Mater. 17: 4577–4593.
  7. Khan S., Gupta A., Verma N. C., Nandi C. K., (2015), Kinetics of protein adsorption on gold nanoparticle with variable protein structure and nanoparticle size. J. Chem. Phys. 143: 164701 –16470 9.
  8. Taylor R. E., (1991), Protein Immobilization Fundamentals and Applications, Marcel Dekker, New York, p. 2.
  9. Shuang L., Xiaofeng Y., Shuai Y., Muzi Z., Xiaoning W., (2012), Technology prospecting on enzymes: Application, marketing and engineering. Comput. Struc. Biotechnol. J.  2: 1–10.
  10. Bornhorst J. A., Falke J. J., (2000), Purification of proteins using polyhistidine affinity tags. In Methods in enzymology. Academic Press. 326: 245-254.
  11. Sharma N. M., Kumar S., Sawhney S. K., (2003), A novel method for the immobilization of tyrosinase to enhance stability. Biotechnol. Applied Biochem. 38: 137-141.
  12. Gooding J. J., Hall E. A. H., (1996), Membrane properties of acrylate bulk polymers for biosensor applications. Biosens. Bioelectron. 11: 1031-1040.
  13. Yildiz A., Gür A., (2007), Adsorption of phenol and chlorophenols on pure and modified sepiolite. J. Serbian Chem. Soc. 72: 467-474.
  14. Adeogun A. I., Kareeem S. O., Adebayo O. S., Balogun S. A., (2017), Comparative adsorption of amylase, protease and lipase on ZnFe2O4: Kinetics, isothermal and thermodynamics studies. 3 Biotechnol. 7: 198-203.
  15. Lagergren S., (1898), About the theory of so-called adsorption of soluble substances. Kungligasvenska Vetenskaps Akademiens. Handlingar Band.24: 1–39.
  16. Ho Y. S., Ng J. C. Y., McKay G., (2000), Kinetics of pollutant sorption by biosorbents: Review. Sep. Purif. Methods. 29: 189–232.
  17. Badruzzaman M., Westerhoff P., Knappe D. R. U., (2004), Intraparticle diffusion and adsorption of arsenic onto granular ferric hydroxide (GFH). Water Res. 38: 4002-4012.
  18. Meissner J., Prause A., Bharti B., Findenegg G. H., (2015), Characterization of protein adsorption onto silica nanoparticles: Influence of pH and ionic strength. Colloid Polym. Sci. 293: 3381-3391.
  19. Wang H., Yuan X., Wu Y., Huang H., Zeng G., Liu Y., Wang X., Lin N., Qi Y., (2013), Adsorption characteristics and behaviors of graphene oxide for Zn (II) removal from aqueous solution. Appl. Surf. Sci. 279: 432-440.
  20. Daoud F. B. O., Kaddour S., Sadoun T., (2010), Adsorption of cellulose Aspergillus niger on a commercial activated carbon: Kinetics and equilibrium studies. Colloids Surf. B: Biointerf. 75: 93–99.