Comparative analysis of antibacterial activity of Silver nanoparticles synthesized using leaf extract of wheat varieties

Document Type: Reasearch Paper

Authors

Department of Biotechnology, Guru Nanak Girls College, Ludhiana, INDIA

10.7508/ijnd.2016.02.005

Abstract

In the present study, we have reported the biological synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract prepared by hot percolation treatment of wheat varities (PBW343, Triticum durum and Aegilops tauschii )for the reduction of silver ions to silver nanoparticles. Bioreduction of Ag+ to Ag0 was observed when aqueous extract augmented with silver nitrate (AgNO3) was incubated for 2 hours at 1000C. The formation of silver nanoparticles was confirmed by surface plasmon resonance as determined by UV-Vis spectra at 430 nm. Silver nanoparticles of size ranging from 5-14 nm of spherical shape were characterized using transmission electron microscopy (TEM). These nanoparticles were found to possess potential antibacterial activity against (Escherichia coli, Staphylococcus aureus, Klebsiella) using disc diffusion method and macro dilution(tube)broth method. This environmental friendly method provides a simple, easy, fast and cost effective method for nanoparticles synthesis and can be used in several areas of medicines and industries.

Keywords

Main Subjects


[1] Maheshwari R. U., Prabhu A. L., Nandagopalan Anburaja V., (2012), Green Synthesis of Silver nanoparticles by using Rhizome Extract of Dioscorea oppositifolia L. and their antimicrobial activity against human pathogens.  IOSR J. Pharm. Biolog. Sci. 1: 2278-2285.
[2] Geoprincy G., Vidhaya B. N. SRRI, Poonghuzhali U., Gandhi N. N., Regenthan S., (2013),  A review on green synthesis of silver nanoparticles.  Asian J. Pharm. Clin. Res. 6: 2438-2441.
[3] Prabhu S., Poulose E. K.., (2012), Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int. Nano Lett. 2: 32-38.
[4] Yasin S., Liu L., Yao J., (2013), Biosynthesis of Silver nanoparticles by Bamboo leaves extract and their antimicrobial activity. J.  Fiber Bioengin. Informat. 6: 77-84.
[5] Banerjee P., Satapathy M., Mukhopahayay A., Das P., (2014), Leaf extract mediated green synthesis of Silver nanoparticles from widely available Indian plants synthesis, characterization, antimicrobial property and toxicity analysis. Springer Open J. 1: 3-8.
[6]Komal R., Arya V., (2013), Biosynthesis and characterization of silver nanoparticles from aqueous leaf extract of Carica papaya and its antibacterial activity. Int. J. Nanomater. Biostruct. 1: 2277-2282.
[7] Ghorbani H. R., Safekordi A. A., Attar H., Sorkhabadi S. M. R., (2011),  Biological and Non-biological Methods for Silver nanoparticles synthesis. Chem. Biochem. Eng. 25: 317-326.
[8] Devi N. N., Shankar D. P., Sutha S., (2012), Biomimetic synthesis of silver nanoparticles from an endophytic fungus and their antimicrobial efficacy. Int. J. Biomed. Adv. Res. 5: 637-642.
[9] Krishnamoorthy P., Jayalakshmi T., (2012), Preparation, characterization and synthesis of silver nanoparticles by using phyllanthusniruri for the antimicrobial activity and cytotoxic effects.  J. Chem. Pharm.Res.11: 4783-4794.
[10] Rekha A., Arya V., ( 2013). Biological synthesis of silver nanoparticles from aqueous extract of endophytic fungus Aspergillus terrus and its antibacterial activity.  Int. J.  Nanomater. Biostruc. 2: 35-39.
[11] Azziz A., (2014), Eco-friendly biosynthesis of silver nanoparticles by Aspergillus parasiticus. Digest J. Nanomater. Biostruc. 6: 1485-1492.
[12] Mishra S., Singh B. R., Singh A., Keswani C., Haqvi A. H., Singh H. B., (2014). Biofabricated Silver nanoparticles act as a strong fungicide against bipolaris sorokiniana causing spot blotch disease in wheat. PLoS ONE.  9: 97881-97886.
[13] Dubeya S. P., Lahtinenb M., Sillanpaa M., (2010), Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochem. 45: 1065-1071.
[14] Jiang X. C., Chen W. M., Chen, Xiong S. X., Yu A. B., (2011), Role of temperature in the growth of silver nanoparticles through a synergetic reduction approach. Springer Open J. 6: 32-39.
[15] Hudzicki J., (2009), Kirby Bauer Disk Diffusion Susceptibility Test protocol. American Society for Microbiology. 15: 55-63.
[16] Franklin R., Cockerill M. A., Wikler J. A., Michael N. D., George M., Eliopoulos M., Jane F.,  Dwight J., Hardy D.,  Hecht W., (2012), Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically and approved standard.  64: 2162-2914.