Modification of Glucose biosensor using Pt/MWCNTs electrode and optimization by application of taguchi method

Document Type: Reasearch Paper

Authors

Department of Mechanical Engineering, Ferdowsi University of Mashhad, Mashhad, Iran

10.7508/ijnd.2016.03.006

Abstract

In this paper, multi-wall carbon nanotubes (MWCNTs), gold nanoparticles (GNp) and glucose oxidase (GOD) was developed for the specific detection of glucose. MWCNTs were chemically modified with the H2SO4–HNO3 pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (PAA) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide cross-linking reaction, respectively. A cleaned Pt electrode was immersed in PAA, MWCNTs, cysteamine and GNp, respectively, followed by the adsorption of GOD, assembling the one layer of films on the surface of Pt electrode (GOD/GNp/MWCNTs/Pt electrode) and was used as working electrodes (anode) along with a platinum auxiliary electrode and the reference electrode Ag/AgCl (cathode). Working electrode was containing the Phosphate-buffered saline (PBS) with PH = 4, 6 and 8 enzyme. Glucose concentration and PBS pH design has been tested and analyzed by QUALTEK-4 software measure. According to the performed experiments and software analysis, with increasing concentration, the flow rate of current production is increased and pH deviance from neutral range reduces the flow. Optimal conditions was obtained in concentrations 1 mmol/lit and pH =6, respectively. After confirmation tests in optimum conditions, the rate of production was obtained, 21.67 mA, which with respect to the expected error rate of application, it was calculated to be 8.1% . This error rate demonstrates that the accuracy of tests is with high sensitivity and accuracy.

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[1] Xia Y., Xiong Y., Lim B., Sharabalak S., (2009), Shape-controlled synthesis of metal nanocrystals: Simple chemistry meets complex physics. Angew. Chem. 48: 60-103.

[2] Tasviri M., Rafiee-Pour H. A., Ghourchian H., Gholami M., (2011), Amine functionalized TiO2–carbon nanotube composite: Synthesis, characterization and application to glucose biosensing. Appl. Nanosci. 1: 89-195.

[3] Zhu J., Kamiya A.,  Yamada T.,  Shi W., Naganuma K., Mukai K., (2002), Surface tensionwettability and reactivity of molten titanium in Ti/yttria-stabilized zirconia system. Mat. Sci. Engin. A. 327: 117-127.

[4] Bahr J. L., Edward T. M., Michael J. B., Smalley R. E., Tour J. M., (2001), Amperometric glucose biosensors based on Prussian Blue and polyaniline–glucose oxidase modified electrodes. Royal Soc. Chem. 15: 193-200.

[5] Pang X., He D., Luo Sh., (2009), An amperometric glucose biosensor fabricated with Pt nanoparticle-decorated carbon nanotubes/TiO2 nanotube arrays composite. Sensors and Actuators. B. 137: 134-138.

[6] Balasubramanian K., Burghard M., (2006), Biosensors based on carbon nanotubes. Anal. Bioanal. Chem. 385: 452-468.

[7] Niraj S., Jiazhi M., Yeow J. T. W., (2006), Carbon nanotube-based sensors.  J. Nanosc. Nanotech. 6: 573-590.

[8] Xi SH., Shi T., Liu D., (2013), Integration of carbon nanotubes to three-dimensional C-MEMS for glucose. Sensors and Actuators A. 198: 15-20.

[9] Odaci D., Timur S., Telefoncu A., (2008), Bacterial sensors based on chitosan matrices. Sensors and Actuators B. 134:   89-94.

[10] Antiochia R., Gorton L., (2007), Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages. Biosens. Bioelectron. 22: 2611-2617.

[11] Odaci D., Timur S., Telefoncu A, (2008), Pyranose oxidase biosensor based on carbon nanotube (CNT)-modified carbon paste electrodes. Sensors and Actuators B. 132: 159-165.

[12] Fan Y., Ryu K.Y. Shin H.K., (2007), Study of glucose oxidase-l-lecithin Langmuir film formation on air–water interface. Current Appl. Phys. 7: 490-495.

[13] Anik U., Cubukcu M., (2008), Examination of the Electroanalytic Performance of Carbon Nanotube (CNT) Modified Carbon Paste Electrodes as Xanthine Biosensor Transducers. Turk. J. Chem. 32: 711-719.

[14] Ivnitski D., Branch B., Atanassov P., (2006), Glucose oxidase anode for biofuel cell based on direct electron transfer. Electrochem. Communic. 8: 1204–1210.

[15] Yoo E. H., Lee S. Y., (2010), Glucose biosensors: An overview of use in clinical practice. Sensors. 10: 4558-4576.

[16] Timur S., Yigzaw Y., Gorton L., (2006), Electrical wiring of pyranose oxidase with osmium redox polymers. Sensors and Actuators B. 113: 684-691.

[17] Upadhye R. A., (2012), Optimization of sand casting Process parameter using taguchi method in foundry. Int. J. Engin. Res. Technol. 7 :1-11.

[18] Madaeni S. S., Koocheki S., (2006), Application of taguchi method in the optimization of wastewater treatment using spiral-wound reverse osmosis element. Chem. Engin. J. 119 : 37-40

[19] Farahbakhsh A., Taghizadeh M., Yakhchali B., Movagharnejad K., (2011), Stabilization of heavy oil-water emulsions using a bio/chemical emulsifier mixture. Chem. Engin. Tech. 34: 1807-1812.

[20] Mehravar R., Jahanshahi M., Najafpour Gh. D., Saghatoleslami N., (2011), Applying the taguchi method for optimized fabrication of  lactalbumin nanoparticles as carrier in drug delivery and food science. Iranica J. Energy & Environm. 2: 87-91.

[21] Ramezani O., Farahbakhsh  A., (2015), The obtain optimum production conditions for Glucose Oxidase biosensor using software Qualtek-4. Int. J. Nano Dimens. 6: 23-30.