Fabrication of coated - collagen electro-spun PHBV nanofiber film by chemical method and its cellular study

Document Type: Reasearch Paper

Authors

1 Department of tissue engineering, Faculty of advance technologies, Tehran university of medical sciences ,Tehran ,Iran.

2 Department of biomedical engineering, Science and research branch-Islamic azad university,Tehran, Iran.

3 Stem cell preparation unit, Eye research cente , Farabi eye hospital,Tehran university of medical sciences, Tehran, and Clinical proteomics research center, Faculty of paramedical sciences, Shahid beheshti university of medical sciences,Tehran, Iran.

4 Department of chemistry, Islamic azad university-tonekabon branch, Iran.

10.7508/ijnd.2010.03.008

Abstract

Tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. Scaffolding Poly Hydroxy Butyrate Valraty (PHBV) has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. One of the important methods for designing nanofiber scaffold is the electrospinnig method. In this study, PHBV nanofibers were well designed; then, modified with the immobilized collagen via the chemical method. The samples were evaluated by ATR-FTIR, SEM, contact angle and finally, cell culture. ATR-FTIR structural analysis showed the presence of collagen on the nanofiber surfaces. The SEM images showed the size average of nanofibers as to be about 280 nm; that increased with a collagen coating up to 400 nm. Contact angle analysis showed 67 degree for uncoated nanofibers and 52 degree for coated nanofibers. Cellular investigations (USS cells) showed better adhesion and cell growth and proliferation of coated samples than uncoated samples.

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